Connect the sequence change to the intended effect
Determine whether sequence correction is the relevant intervention and specify the edit, biological context and validation question.
Editing approaches under consideration
- CRISPR editing
- Base editing
- Prime editing
The foundations and the work ahead
- In BioTwin today · CRISPR, base editing and prime editing belong to the research vision. Variant and genome representations provide related foundations.
- Development and validation · A complete editing workflow would need guide/editor design, off-target assessment, delivery and experimental validation. These are future modality-specific capabilities.
Requirements for an editor-specific program
Make the intended sequence change and causal biological hypothesis explicit. A specialist workflow would need editor and guide design, off-target assessment, delivery and functional validation.
Related biological context
Related research methods to explore; a combined modality-specific workflow is not yet established.
- Isoform-Specific Target Assessment · Determine whether an isoform distinction changes the target, intervention or assay your program should use. Read the method
- Resistance Min-Cut and Protein–RNA Co-Targeting · Investigate modeled signaling escape routes before choosing a protein–RNA co-targeting hypothesis. Read the method